Finding IQR of groups of rows
iqr, r
Solution
It's not clear if your problem is about aggregating, or about your (??) definition of IQR. There are many ways to calculate IQR (see this and this). As far as I can tell, none of them yield the results in your post.
As far as aggregating based on spot size and molarity, here are two ways:
# use aggregate(...) in base R - will be slow with large datasets
aggregate(Cy3_Fluorescence~Molarity+Spot_Size,df,IQR)
# Molarity Spot_Size Cy3_Fluorescence
# 1 5 100pl 478.5
# 2 10 100pl 576.0
# 3 25 100pl 65.0
# 4 50 100pl 221.0
# 5 100 100pl 129.0
# use data.table - will be extremely fast.
library(data.table)
setDT(df)[,list(IQR=IQR(Cy3_Fluorescence)),by=list(Molarity,Spot_Size)]
# Molarity Spot_Size IQR
# 1: 100 100pl 129.0
# 2: 50 100pl 221.0
# 3: 25 100pl 65.0
# 4: 10 100pl 576.0
# 5: 5 100pl 478.5
Problem
I am wanting to find the IQR of a range of values in a dataframe. These values are also grouped, therefore I need to find the IQR of each group in the dataframe. I have the following table: ``` Block DNAname Spot_Size Molarity Cy3_Fluorescence 1 DNA 01 100pl 100 14266 1 DNA 01 100pl 100 16020 1 DNA 01 100pl 100 15705 1 DNA 01 100pl 100 15783 1 DNA 01 100pl 100 15834 1 DNA 01 100pl 50 12248 1 DNA 01 100pl 50 12209 1 DNA 01 100pl 50 12511 1 DNA 01 100pl 50 12316 1 DNA 01 100pl 50 12469 1 DNA 01 100pl 25 9626 1 DNA 01 100pl 25 9804 1 DNA 01 100pl 25 9794 1 DNA 01 100pl 25 10020 1 DNA 01 100pl 25 9739 1 DNA 01 100pl 10 7158 1 DNA 01 100pl 10 6802 1 DNA 01 100pl 10 7378 1 DNA 01 100pl 10 5949 1 DNA 01 100pl 10 7484 1 DNA 01 100pl 5 5257 1 DNA 01 100pl 5 5560 1 DNA 01 100pl 5 6076 1 DNA 01 100pl 5 5925 ``` I run the following code to find the IQR: ``` aggregate(Cy3.DNA1.100pl.1uM$Cy3_Fluorescence, list(Molarity= Cy3.DNA1.100pl.1uM$Molarity, Spot_Size=Cy3.DNA1.100pl.1uM$Spot_Size ), IQR) ``` This gives me the ouput: ``` Molarity Spot_Size x 5 100pl 384 10 100pl 576 25 100pl 65 50 100pl 221 100 100pl 129 ``` This output groups all the molarities correctly but the IQR is not correct. If the code above has mean as a function instead of IQR the value for x (the function value) is correct as so: ``` Molarity Spot_Size x 5 100pl 5752.4 10 100pl 6954.2 25 100pl 9796.6 50 100pl 12350.6 100 100pl 15521.6 ``` The expected IQRS should be as follows: ``` Molarity IQR 100 324.25 50 258 25 363 10 519.5 5 400 ``` Any help would be much appreciated. If any one has any ideas how I could perform this function for IQR, when there are groups of spot sizes (where spot size ranges from 100pl-400pl) including the molarity categories I would like to hear them. Thank you kindly SO.